A Specialized Company Providing a Variety of Monoclonal Antibodies Against Antigens of Veterinary, Medical, and Diagnostic Significance :
(A full list of monoclonals available may be found in Linscott's Directory)
Feline Leukemia Virus (gp70 env; p15e tm, and p27 gag)
Feline Immunodeficiency Virus (gp100 env; gp41 tmp; p24 gag; Vif; integrase; dUTP'ase, and protease)
Feline Coronavirus (Feline Infectious Peritonitis Virus)
Feline Herpes Virus (type 1; FVR)
Cat Immunoglobulins: IgM; IgA; IgG, also kappa and lambda light chains.
Cat Lymphocyte Membrane: CD4; CD8; Pan-T; B/T activation marker; B-cell differentiation marker; MHC Class II. Cat Interleukin 2; Cat complement component C'3.
Dog Viruses: Canine Distemper Virus
Canine Adenovirus type 2
Canine Parinfluenza Virus
Dog Parasites: Dirofilaria immitis (Heartworm)
Dog Immunoglobulins: IgE; IgM; IgA; IgG.
Dog Lymphocyte Membrane: CD4; CD8; Pan-T; B/T activation marker; B-cell differentiation marker.
Monoclonal Antibodies (MAbs)
All MAbs supplied by Custom Monoclonals International are derived from fusing immune mouse spleen and mesenteric lymph node cell suspensions with a HAT sensitive mouse myeloma - usually a proprietary subclone of X-63, but occasionally P8.653. The hybridomas are subcloned at least twice and regularly checked for production of specific MAb.
Hybridomas are grown in HAT containing culture medium. The antibodies are purified by ammonium sulfate precipitation and then by isolation over an SpA column (using a propriety buffer system which results in stabilizing the MAbs, and in the absorption of all mouse Ig isotypes and subclasses). The MAbs are eluted from the SpA column by citric acid into Tris buffer pH 8.0, then concentrated by reverse pressure dialysis. The final MAb concentration is established by a spectrophotometer reading at O.D.280 using an extinction coefficient of 1.4. MAbs are supplied in PBS pH 7.2 with 0.05% sodium azide as preservative. MAbs are shipped FedEx at ambient temperature, and laboratory storage at 4oC is recommended.
For ELISA, MAbs are generally effective when used at 1.0 ug/well or less. For FACS most antibodies can be diluted at least 1:500 per 1 X 106 cells, and for Western blot applications I recommend about 15 ug/ml buffer/strip, but optimal conditions for use must be established for each application by the individual investigator.