Antigen: IgM

Monoclonal Designation: CM7.

Ig Isotype/subclass plus light chain: IgG1 kappa.

Specific reactivity: IgM heavy chain Fc5u specific. Equal IgM specific reactivity against IgM of: Human; Monkey (Rhesus and Marmoset, i.e. both Old and New World monkeys); Horse; Cat; Dog; Pig).

Properties:

Western blot: using reducing gels this MAb gives band a major band at 75kD against IgM from the species listed above, but does not react with pure IgA; IgG or light chain. Will bind specifically immune IgM in Western blots when acting as an IgM probe

ELISA: Excellent probe.

Affinity Chromatography: Can be cross-linked to Sepharose-4B to provide an affinity column to isolate IgM, but not as effective as MAb CM6E for this application.

Immunofluoresence: Unknown.

Immunohistocytochemistry: Unknown.

FACS Analysis: Binds IgM producing B-cells only. Independently evaluated on horse B-cells as excellent reagent (ref 4 below).

Preparation and supply: SpA purified from tissue culture supernatant and supplied in PBS pH 7.2 with 0.05% sodium azide as preservative. Biotin conjugated MAb also usually available. Pricing: $(US)425.00 per mg SpA purified; $(US)495.00 per mg SpA purified and biotin conjugated. Minimum order 1.0 mg.

MAb CM7 References:

1. Reubel G. H., George J. W., Barlough J. E., Higgins J., Grant C. K., Pedersen N. C. Interaction of acute Feline Herpesvirus-1 and chronic Feline Immunodeficiency Virus infections in experimentally infected specific pathogen free cats. Vet. Immunol. Immunopathol. 1992; 35: 95-119.

2. Grant C. K. Purification and characterization of feline IgM and IgA isotypes and three subclasses of IgG. In: Feline Immunology and Immunodeficiency, Eds. Willett B. J. and Jarrett O. Oxford University Press 1995, pp 95-107.

3. Grant C.K. Immunoglobulin changes associated with feline immunodeficiency virus infections. In: Feline Immunology and Immunodeficiency, Eds. Willett B. J. and Jarrett O. Oxford University Press 1995, pp 170-189.

4. Lunn D.P. et al. Report of the second equine leucocyte antigen workshop, Squaw Valley, California, July 1995. Vet. Immunol. Immunopathol. 1998; 62: 101-143.

5. Beatty J.A., Lawrence C.E., Callanan J.J., Grant C.K., Gault E.A., Neil J.C. and Jarrett O. Feline immunodeficiency virus-associated lymphoma: a potential role for immune dysfunction in tumourigenesis. Vet. Immunol. Immunopathol. 1998; 65: 309-322.

Other related IgM MAbs available: CM6E