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Antigen: IgA

Monoclonal Designation: IgA9-6A.

Ig Isotype/subclass plus light chain: IgG1 lambda.

Specific reactivity: Cat IgA alpha chain specific. IgA specific reactivity against Cat; some cross-reactivity with IgA from dog, also rhesus and marmoset.

Properties:

Western blot: using reducing gels this MAb gives band a major band at 55 kD against IgA using purified cat IgA, but does not react with pure cat IgM; IgG or light chain. Will bind specifically immune IgA in Western blots when acting as an antigen specific IgA probe.

ELISA: Excellent capture or probe. As capture with IgA5-3B-biotin as probe the sandwich will detect IgA secreted in cat saliva.

Affinity Chromatography: Can be cross-linked to Sepharose-4B to provide an affinity column to isolate IgA, but not as effective as MAb IgA5-3B for this application.

Immunofluoresence: Unknown.

Immunohistocytochemistry: Unknown.

FACS Analysis: Not known.

Preparation and supply: SpA purified from tissue culture supernatant and supplied in PBS pH 7.2 with 0.05% sodium azide as preservative. Biotin conjugated MAb also usually available. Pricing: $(US)425.00 per mg SpA purified; $(US)495.00 per mg SpA purified and biotin conjugated. Minimum order 1.0 mg.

MAb IgA9-6A references:

1. Grant C. K. Purification and characterization of feline IgM and IgA isotypes and three subclasses of IgG. In: Feline Immunology and Immunodeficiency, Eds. Willett B. J. and Jarrett O. Oxford University Press 1995, pp 95-107.

2. Grant C.K. Immunoglobulin changes associated with feline immunodeficiency virus infections. In: Feline Immunology and Immunodeficiency, Eds. Willett B. J. and Jarrett O. Oxford University Press 1995, pp 170-189.

3. Beatty J.A., Lawrence C.E., Callanan J.J., Grant C.K., Gault E.A., Neil J.C. and Jarrett O. Feline immunodeficiency virus-associated lymphoma: a potential role for immune dysfunction in tumourigenesis. Vet. Immunol. Immunopathol. 1998; 65: 309-322.

Other related IgA MAbs available: IgA5-5B CDA2-43