Antigen: Feline IgG

Monoclonal Designation: KIM2-1A.

Ig Isotype/subclass plus light chain: IgG1 kappa.

Specific reactivity: feline IgG, no activity against feline IgM or IgA.

Properties:

Western blot: using reducing gels this MAb gives band a major band at 50.0 kD against purified feline IgG.

ELISA: Excellent probe for antigen specific cat IgG.

Neutralization: Not applicable.

Affinity Chromatography: Not known.

Immunofluoresence: Unknown.

Immunohistocytochemistry: Positive, but result needs confirmation.

FACS Analysis: Not known.

Preparation and supply: SpA purified from tissue culture supernatant and supplied in PBS pH 7.2 with 0.05% sodium azide as preservative. Biotin conjugated MAb also usually available. Pricing: $(US)425.00 per mg SpA purified; $(US)495.00 per mg SpA purified and biotin conjugated. Minimum order 1.0 mg..

MAb Kim2-1A References:

1. Grant C. K. Purification and characterization of feline IgM and IgA isotypes and three subclasses of IgG. In: Feline Immunology and Immunodeficiency, Eds. Willett B. J. and Jarrett O. Oxford University Press 1995, pp 95-107.

Other related IgG specific MAbs available: GPB2-2B, FDG1-2A, FDG1-43A.