Antigen: Dog IgE.

Monoclonal Designation: E2-19A1.

Ig Isotype/subclass plus light chain: IgG1 kappa.

Specific reactivity: Specific for Dog IgE; does not react with dog IgM; IgA, or IgG. Comment: There may be two dog IgE subclasses, if so this MAb is likely specific for only one IgE subclass. Further, if the dog IgE subclasses are anti-allergy and anti-parasite related, respectively, then this MAb is probably an anti-parasite IgE MAb. This MAb appears unrelated to anti-IgE MAb CDE1-6A1.

Properties:

Western blot: with affinity purified dog IgE in reducing gels this MAb gives one major band at about 62-65 kD. Using heartworm larval antigen in a reducing blot, this MAb has demonstrated weak activity to act as a probe to detect anti-heartworm larval antigen specific IgE in recently infected dogs.

ELISA: Not a good MAb for capture, but works effectively with MAb E6-71 (see below) as an ELISA probe.

Skin sensitivity: Will rapidly induce large immediate hypersensitivity wheals if injected into dogs i.d. in ug. or ng. quantities.

Preparation and supply: SpA purified from tissue culture supernatant and supplied in PBS pH 7.2 with 0.05% sodium azide as preservative. Biotin conjugated MAb also usually available. Pricing: $(US)425.00 per mg SpA purified; $(US)495.00 per mg SpA purified and biotin conjugated. Minimum order 1.0 mg.

References: None to date.

Other related anti-dog IgE MAbs available: E6-2A1 E6-59A1 E6-71A1 CDE1-6A1