Antigen: Canine/Feline Parvovirus (Feline Panleucopenia Virus)
Monoclonal Designation: CPV1-2A1. (information updated 10/3/99).
Ig Isotype/subclass plus light chain: IgG2A kappa.
Specific reactivity and cross-reactivity: Positive against Canine and Feline Parvovirus (Feline Panleucopenia Virus)
Negative against Canine Adenovirus (type2); Canine Coronavirus; Canine Distemper Virus, Canine Parinfluenza Virus.
Western blot: gives weak specific bands at 153; 100; 41, and 15 kD in reducing gels with CPV as antigen.
ELISA: Good ELISA probe with polyclonal anti-CPV immune IgG as capture.
Virus neutralization: not known.
Affinity Chromatography: not known.
Immunofluoresence: Positive against CPV.
Immunohistocytochemistry: Excellent for use on paraffin embedded tissues of dogs and cats, appears also to specifically bind parvovirus in tissues of larger cats (positive result obtained on lynx).
FACS Analysis: not known.
Preparation and supply: SpA purified from tissue culture supernatant and supplied in PBS pH 7.2 with 0.05% sodium azide as preservative. Biotin conjugated MAb also usually available. Pricing: $(US)425.00 per mg SpA purified; $(US)495.00 per mg SpA purified and biotin conjugated. Minimum order 1.0 mg.
1) Kipar, A., Kremendahl, J., Grant, C.K., Von Bothmer, I., and Reinacher, M. Expression of viral proteins in feline leukemia virus-associated enteritis. Vet. Pathol. 2000, 37: 129-136.
Other related CPV1-2 MAbs available: CPV1-4 CPV1-9 CPV1-24 31.2 31.3