Antigen: Canine Parainfluenza Virus
(Expected cross-reactivity with anti-human Parainflenza Type 2).

Monoclonal Designation: CPI-1-31. (Following information updated 12/10/01).
MAb is probable anti-core specific.

Ig Isotype/subclass plus light chain: IgG2B kappa

Specific reactivity and cross-reactivity: Positive against Canine Parainfluenza Virus.
Negative against Canine Adenovirus (type2); Canine Coronavirus; Canine Distemper Virus; Canine Parvovirus; Feline Leukemia Virus, and Feline Immunodeficiency Virus.

Properties:

Western blot: not known.

ELISA: Excellent ELISA capture or probe in combination with polyclonal (goat) anti-CPI immune IgG.

Virus neutralization: not known.

Affinity Chromatography: not known.

Immunofluoresence: Very strong positive against CPI: positive on discrete cytoplasmic inclusions of varying size

Immunohistocytochemistry: not known.

FACS Analysis: not known.

Preparation and supply: SpA purified from tissue culture supernatant and supplied in PBS pH 7.2 with 0.05% sodium azide as preservative. Biotin conjugated MAb also usually available.Pricing: $(US)425.00 per mg SpA purified; $(US)495.00 per mg SpA purified and biotin conjugated. Minimum order 1.0 mg.

CPI-1-31A1 References:

None.

Other related CPI specific MAbs available:

CPI-2-2A1 (IgG2A kappa). IFA: Some CPI specific diffuse cytoplasmic staining with stronger perinuclear inclusion reaction. Reaction against different epitope than CPI-1-31A1, and maybe against a different viral antigen.