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Antigen: Feline/Canine Coronavirus. Monoclonal Designation: CCV2-2 Ig Isotype/subclass plus light chain: IgG1 kappa. Specific reactivity and cross-reactivity: Positive against CCV and FIPV. Negative against Canine Coronavirus; Canine Distemper Virus; Canine Parvovirus; Canine Parinfluenza Virus. Properties: Western blot: gives weak specific bands at 56, 46 and 40 kD in reducing gels with CCV as antigen. Western blot against FIPV not known. ELISA: Excellent ELISA probe with FIPV3-70 as capture. Virus neutralization: not known. Affinity Chromatography: not known. Immunofluoresence: Positive against CCV and FIPV; not known against TGEV. Immunohistocytochemistry: Not known. FACS Analysis: not known. Preparation and supply: SpA purified from tissue culture supernatant and supplied in PBS pH 7.2 with 0.05% sodium azide as preservative. Biotin conjugated MAb also usually available. Pricing: $(US)425.00 per mg SpA purified; $(US)495.00 per mg SpA purified and biotin conjugated. Minimum order 1.0 mg. CCV2-2 References: None. Other related CCV2-2 MAbs available: Several, but in particular MAb FIPV3-70 works effectively as a capture with CCV2-2-biotin as probe to provide a sensitive MAb/MAb ELISA for CCV in biological fluids. |