Antigen: Feline Leukemia Virus (FeLV) p15e transmembrane.

Monoclonal Designation: PF6J-2A1

Ig Isotype/subclass plus light chain: IgG2A kappa.

Specific reactivity: FeLV transmembrane, mapping towards the amino-terminal domain.

Properties:

Western blot: using ultracentrifuge precipitated FeLV in reducing gels this MAb gives bands at 20 kD and 15 kD. The former is the entire FeLV p15e, including the intracellular sequence, and the latter is the truncated p15e sheared from the cell membrane surface.

ELISA: Excellent probe with goat anti-gp70 capture.

FeLV neutralization: Not known.

Affinity Chromatography: Not known.

Immunofluoresence: Positive.

Immunohistocytochemistry: Effective in paraffin embedded tissue to detect FeLV tmp p15e.

FACS Analysis: Not known.

Preparation and supply: SpA purified from tissue culture supernatant and supplied in PBS pH 7.2 with 0.05% sodium azide as preservative. Biotin conjugated MAb also usually available. Pricing: $(US)425.00 per mg SpA purified; $(US)495.00 per mg SpA purified and biotin conjugated. Minimum order 1.0 mg..

MAb PF6J-2A References:

1) Kovacevic, S., Kipar, A., Kremendahl, J., Teebken-Schuler, D., Grant, C.K., and Reinacher, M. Immunohistochemical diagnosis of feline leukemia virus infection in formalin-fixed tissue. Eur. J. Vet. Path. 1997, 3: 13-19.

2) Burns, C.C., Poss, M.L., Thomas, E., and Overbaugh, J. Mutations within a putative cysteine loop of the transmembrane protein of an attenuated immunodeficiency-inducing feline leukemia virus variant inhibit envelope protein processing. J. Virol. 1995, 69: 2126-2132.

3) Kipar, A., Kremendahl, J., Grant, C.K., Von Bothmer, I., and Reinacher, M. Expression of viral proteins in feline leukemia virus-associated enteritis. Vet. Pathol. 2000, 37: 129-136.

Other related FeLV tmp 15e MAbs available: K5B2: by competitive inhibition this MAb maps even further towards the amino-terminal gp70 junction than PF6J-2A.