Antigen: Feline Leukemia Virus (FeLV) p27 core.

Monoclonal Designation: PF12J-10A.

Ig Isotype/subclass plus light chain: IgG1 kappa.

Specific antigen reactivity: FeLV p27.

Properties:

Western blot: using ultracentrifuge precipitated FeLV in reducing gels gives strong bands at about gpr 80 kD (glycosylated FeLV gag gene product, p10+p12+p15+p27), then several bands representing assorted partial gag gene products, then a strong band at p28.5 (p27), and perhaps one or two weak bands >27kD (p27 degradation products).

ELISA: Good capture, excelent probe when used with goat anti-FeLV p27 specific IgG

FeLV neutralization: None.

Affinity Chromatography: Cross-linked to Sepharose-4B this MAb provides an excellent affinity column to isolate FeLV p27.

Immunofluoresence: Positive.

Immunohistocytochemistry: Effective in paraffin embedded tissue to detect FeLV p27 core.

FACS Analysis: Not known Preparation and supply: SpA purified from tissue culture supernatant and supplied in PBS pH 7.2 with 0.05% sodium azide as preservative. Biotin conjugated MAb also usually available.Pricing: $(US)425.00 per mg SpA purified; $(US)495.00 per mg SpA purified and biotin conjugated. Minimum order 1.0 mg.

PF12J-10A References:

1. Kovacevic, S., Kipar, A., Kremendahl, J., Teebken-Schuler, D., Grant, C.K., and Reinacher, M. Immunohistochemical diagnosis of feline leukemia virus infection in formalin-fixed tissue. Eur. J. Vet. Path. 1997, 3: 13-19.

2. Kipar, A., Kremendahl, J., Addie, D.D., Leukert, W., Grant, C.K., and Reinacher, M. Fatal enteritis associated with coronavirus infection in cats. J. Comp. Path. 1998, 119: 1-14.

3) Kipar, A., Kremendahl, J., Grant, C.K., Von Bothmer, I., and Reinacher, M. Expression of viral proteins in feline leukemia virus-associated enteritis. Vet. Pathol. 2000, 37: 129-136.