Antigen: Feline Immunodeficiency Virus (FIV) p24 gag.

Monoclonal Designation: PAK3-2C1.

Ig Isotype/subclass plus light chain: IgG1 kappa.

Specific antigen reactivity: FIVp24 gag.

MAb is directed to an immunodominant p24 epitope available on the final FIV p24 gene product and several higher M. Wt. p24 precursors. PAK3-2C1 competitively inhibits binding of several other anti-p24 specific MAbs, indicating high affinity. Negative against Feline Leukemia Virus; Feline Herpes Virus type 1; Feline Coronavirus and Feline Calicivirus.

Properties:

Western blot: in reducing gels, and using ultracentrifuge precipitated FIV, this MAb gives bands at about 50; 24(very strong) and 16 kD.

ELISA: Excellent capture with polyclonal probes such as rabbit anti-p24 (synthetic peptide) or FIV infected cat serum (using appropriate anti-IgG enzyme conjugated tertiary antibodies).

FIV neutralization: Probably negative.

Affinity Chromatography: Antigen affinity probably too high for useful purification of FIV p24.

Immunofluoresence: Positive?

Immunohistocytochemistry: Excellent on methanol fixed FIV positive cell monolayers, but minimally effective in paraffin embedded tissue to detect FIV p24. Excellent on frozen sections.

FACS Analysis: Positive? Preparation and supply: SpA purified from tissue culture supernatant and supplied in PBS pH 7.2 with 0.05% sodium azide as preservative. Biotin conjugated MAb also usually available. Pricing: $(US)425.00 per mg SpA purified; $(US)495.00 per mg SpA purified and biotin conjugated. Minimum order 1.0 mg.

PAK3-2C1 References:

1) Lerner D.L., Wagman P.C., Phillips T.R., Prospero-Garcia O., Henriksen S.J., Fox H.S., Bloom F.E. and Elder J.H. Increased mutation frequency of feline immunodeficiency virus lacking functional deoxyuridine-triphosphatase. P.N.A.S. (USA) 1995; 92: 7480-7484.

2) Song W., Collisson E. W., Li J., Wolf A. M., Elder J. H., Grant C. K., Brown W. C. Feline Immunodeficiency Virus (FIV)-specific cytotoxic T lymphocytes from chronically infected cats are induced in vitro by retroviral vector-transduced feline T cells expressing the FIV capsid antigen. Virology 1995; 209: 390-399.

3) Li J., Brown W. C., Song W., Carpino M. R., Wolf A.. M., Grant C. K., Elder J. H., Collisson E.W. Retroviral vector-transduced cells expressing the core polyprotein induce feline immunodeficiency virus specific cytotoxic T lymphocytes from infected cats. Virus Research 1995, 38: 93-109.

4) Simon, J. H. M., Fouchier, R. A. M., Southerling, T. E., Guerra, C. B., Grant, C. K., and Malim, M. H. The vif and gag proteins of human immunodeficiency virus type-1 colocalize in infected human T-cells. J. Virol. 1997, 71: 5259-5267.

5) Chatterji, U., Grant, C.K., and Elder, J.E. Feline immunodeficiency virus Vif localizes to the nucleus. J. Virol. 2000, 74: 2533-2540.

Other related FIV p24 MAbs available:

1) PAK1-2B2. This MAb acts as an ELISA probe with PAK3-2C1 as capture. No competitive inhibition occurs with this MAb, probably because PAK1-2B2 appears to bind to the carboxyterminal 1 kD segment of FIV p24 which is cleaved during the final maturation step of the FIV p24 gene product.

2) Several anti-FIV p24 specific MAbs, none of which strongly bind to the FIV p24 precursors, but all of which bind FIV p24. All MAbs in this series are competitively inhibited by PAK3-2C1.