Antigen: Feline/Canine/Porcine Coronavirus.

Monoclonal Designation: FIPV3-70.

Ig Isotype/subclass plus light chain: IgG2A kappa.

Specific reactivity: Positive against FIPV; CCV; TGEV, and Ferret Coronavirus. Negative against Canine Adenomavirus type 2; Canine Distemper Virus; Canine Parvovirus; Canine Parinfluenza Virus.

Specific reactivity and cross-reactivity:Positive against CCV, TGEV, FPIV, and Ferret Coronavirus. Negative against: Feline Leukemia Virus; Feline Immunodeficiency Virus; Feline Calicivirus; Feline Herpies Virus; Canine Adenovirus (type 2); Canine Distemper Virus; Canine Parvovirus; Canine Parinfluenza Virus.

Properties:

Western blot: gives strong specific band at 56 and 46 kD in reducing gels with CCV as antigen.

Apparently does not Western blot against FIPV.

Western blot against TGEV not known.

ELISA: Excellent ELISA capture or probe.

Virus neutralization: not known.

Affinity Chromatography: not known.

Immunofluoresence: Positive against CCV and FIPV (type 1 and type 2); not known against TGEV. Versus FIP shows intense cytoplasmic staining; zero nuclear staining, and minimal membrane activity.

Immunohistochemistry: Effective in paraffin embedded tissue to detect CCV; TGEV, and FIPV.

FACS Analysis: not known.

Preparation and supply: SpA purified from tissue culture supernatant and supplied in PBS pH 7.2 with 0.05% sodium azide as preservative. Biotin conjugated MAb also usually available. Pricing: $(US)425.00 per mg SpA purified; $(US)495.00 per mg SpA purified and biotin conjugated. Minimum order 1.0 mg.

FIPV3-70 References:

1. Kipar, A., Kremendahl, J., Addie, D.D., Leukert, W., Grant, C.K., and Reinacher, M. Fatal enteritis associated with coronavirus infection in cats. J. Comp. Path. 1998, 119: 1-14.

2. Kipar A, Bellmann S., Kremendahl J., Kohler K. and Reinacher M. Cellular composition, coronavirus antigen expression and production of specific antibodies in lesions in feline infectious peritonitis. Vet. Immunol. Immunopathol. 1998; 65: 243-257.

3) Kipar, A., Kremendahl, J., Grant, C.K., Von Bothmer, I., and Reinacher, M. Expression of viral proteins in feline leukemia virus-associated enteritis. Vet. Pathol. 2000, 37: 129-136.

Other related FIPV3-70 MAbs available:

Several, but in particular MAb CCV2-2-biotin works effectively as a probe with FIPV3-70 as a capture to provide a sensitive MAb/MAb ELISA for coronavirus in canine biological fluids.