Antigen: Feline Immunodeficiency Virus (FIV) gp105 env.

Monoclonal Designation: CE4-13B1.

Ig Isotype/subclass plus light chain: IgG2A kappa.

Specific antigen reactivity: FIV gp 105 env.

MAb is directed to a gp105 epitope, cross-reactive with Petaluma; 34TF10; Ppr and and FIV isolate from Europe. Negative against Feline Leukemia Virus; Feline Herpes Virus type 1; Feline Coronavirus and Feline Calicivirus.

Properties:

Western blot: in reducing gels, and using ultracentrifuge precipitated FIV, this MAb gives bands a band at gp105, and another at about 25 kD.

ELISA: Excellent probe with MAbs CE4-8A1B or CE4-9 to capture FIV env.

FIV neutralization: Not known.

Affinity Chromatography: Good to affinity purify FIV env from culture supernatant.

Immunofluoresence: Positive?

Immunohistocytochemistry: Not known.

FACS Analysis: Positive. Preparation and supply: SpA purified from tissue culture supernatant and supplied in PBS pH 7.2 with 0.05% sodium azide as preservative. Biotin conjugated MAb also usually available. Pricing: $(US)425.00 per mg SpA purified; $(US)495.00 per mg SpA purified and biotin conjugated. Minimum order 1.0 mg.

CE4-13B1 References:

None Other related FIV gp105 MAbs available:

1) CE4-8A1B

2) CE4-9