Antigen: Feline Leukemia Virus (FeLV) gp85/gp70 envelope.

Monoclonal Designation: C1G10 (Following information updated 7/21/01).

Ig Isotype/subclass plus light chain: IgG2A kappa
Specific reactivity: FeLV subgroup B and C specific. No reactivity with FeLV subgroup A. C1G10 neutralizes FeLV subgroups B and C equally. The C1G10 binding epitope binds downstream (or carboxyterminal) of aa 214 – 218 (MGPNL), probably in the aa 300 to 500 region.

Properties:

Western blot: not a good reagent in reducing gels, probably because the MAb binds to a conformational epitope (C1G10 binds FeLV gp70 subgroup B or C and hence blocks virus binding to the appropriate cell membrane receptor). Nevertheless, C1G10 will give weak bands at 85 and 70 kD with high concentrations of FeLV subgroup B or C envelope.

ELISA: Excellent probe with goat anti-gp70 capture.

FeLV neutralization: Specifically neutralizes FeLV subgroups B and C equally.

Affinity Chromatography: Can be cross-linked to Sepharose-4B to provide an affinity column to isolate

FeLV gp70 (this gp70 is assumed to be subgroup B/C specific).

Immunofluoresence: Positive

Immunohistocytochemistry: Not known.

Immunoprecipitation: Not known.

FACS Analysis: Positive.

Preparation and supply: SpA purified from tissue culture supernatant and supplied in PBS pH 7.2 with 0.05% sodium azide as preservative. Biotin conjugated MAb also usually available. Pricing: $(US)425.00 per mg SpA purified; $(US)495.00 per mg SpA purified and biotin conjugated. Minimum order 1.0 mg.

MAb C1G10 References:

1) Grant C. K., Ernisse B. J., Jarrett O., Jones F. R. Feline leukemia virus envelope gp70 of subgroups B and C defined by monoclonal antibodies with cytotoxic and neutralizing functions.
J. Immunol. 1983; 131: 3042-3048.