Antigen: Feline Leukemia Virus (FeLV) gp85/gp70 envelope.

Monoclonal Designation: C11D8

Ig Isotype/subclass plus light chain: IgG2B kappa.

Specific reactivity: aa 214 to 218 in gp70 sequence, i.e. MGPNL. (MAb may weakly cross-react with aa sequence MGPLL located in the intracellular portion of FeLV p15e transmembrane protein to give the 20 kD band in Western blots).

Properties:

Western blot: using ultracentrifuge precipitated FeLV in reducing gels this MAb gives bands at 85; 70; 45 (weak); 33, and 20 kD.

ELISA: Excellent probe with goat anti-gp70 capture. FeLV neutralization: Neutralizes all three FeLV subgroups (A, B, and C) equally.

Affinity Chromatography: Can be cross-linked to Sepharose-4B to provide an affinity column to isolate FeLV gp70.

Immunofluoresence: Positive Immunohistocytochemistry: Effective in paraffin embedded tissue to detect FeLV envelope.

FACS Analysis: Positive.

Preparation and supply: SpA purified from tissue culture supernatant and supplied in PBS pH 7.2 with 0.05% sodium azide as preservative. Biotin conjugated MAb also usually available. Pricing: $(US)425.00 per mg SpA purified; $(US)495.00 per mg SpA purified and biotin conjugated. Minimum order 1.0 mg.

MAb

C11D8 References: 1) Grant C. K., Ernisse B. J., Jarrett O., Jones F. R. Feline leukemia virus envelope gp70 of subgroups B and C defined by monoclonal antibodies with cytotoxic and neutralizing functions. J. Immunol. 1983; 131: 3042-3048.

2) Abkowitz J. L., Holley R. D., Grant C. K. Retrovirus-induced feline pure red cell aplasia. Hematopoietic progenitors are infected with feline leukemia virus and erythroid burst forming cells are uniquely sensitive to heterologous complement. J. Clin. Invest. 1987; 80: 1056-1063.

3) Elder J. H., McGee J. S., Munson M., Houghten R. A., Kloetzer W., Bittle J. L. Grant C. K. Localization of neutralizing regions of the envelope gene of feline leukemia virus by using anti-synthetic peptide antibodies. J. Virol. 1987; 61: 8-15.

4) Nicolaisen-Strouss K., Kumar H. P. M., Fitting T., Grant C. K., Elder J. H. Natural feline leukemia virus variant escapes neutralization by a monoclonal antibody via an amino acid change outside the antibody-binding epitope. J. Virol. 1987; 61: 3410-3415.

5) Sheets R. L., Pandey R., Klement V., Grant C. K., Roy-Burman P. Biologically selected recombinants between Feline Leukemia Virus (FeLV) subgroup A and an endogenous FeLV element. Virology, 1992; 190: 849-855.

6) Kovacevic, S., Kipar, A., Kremendahl, J., Teebken-Schuler, D., Grant, C.K., and Reinacher, M. Immunohistochemical diagnosis of feline leukemia virus infection in formalin-fixed tissue. Eur. J. Vet. Path. 1997, 3: 13-19.

7) Burns, C.C., Poss, M.L., Thomas, E., and Overbaugh, J. Mutations within a putative cysteine loop of the transmembrane protein of an attenuated immunodeficiency-inducing feline leukemia virus variant inhibit envelope protein processing. J. Virol. 1995, 69: 2126-2132.

8) Rohn, J.L., Moser, M.S., Gwynn, S.R., Baldwin, D.N., and Overbaugh, J. In vivo evolution of a novel, syncytium-inducing and cytopathic feline leukemia virus variant. J. Virol. 1998, 72: 2686-2696.

9) Moser, M., Burns, C.C., Boomer, S., and Overbaugh, J. The host range and interference properties of two closely related feline leukemia variants suggest that they use distinct receptors. Virol. 1998, 242: 366-377.

10) Kipar, A., Kremendahl, J., Grant, C.K., Von Bothmer, I., and Reinacher, M. Expression of viral proteins in feline leukemia virus-associated enteritis. Vet. Pathol. 2000, 37: 129-136.

Other related FeLV gp70 MAbs available:

C1G10: IgG2A kappa; specifically neutralizes FeLV subgroups B and C equally.

G13D8: IgG2B kappa; specifically neutralizes FeLV subgroup C only.